uuo group  (TargetMol)

Journal: Scientific Reports

Article Title: UHRF1 promotes epithelial-mesenchymal transition mediating renal fibrosis by activating the TGF-β/SMAD signaling pathway

doi: 10.1038/s41598-025-86496-9

Figure Lengend Snippet: Hinokitiol could inhibit the expression of UHRF1 in HK-2 cells. ( A ) Molecular formula and synonym of Hinokitiol. ( B ) Cell viability was evaluated by CCK-8 assay after treatment with different concentrations of Hinokitiol in HK-2 cells for 24 h or 48 h. ( C ) The RNA expression level of UHRF1 was assessed by real-time polymerase chain reaction in HK-2 cells treated with Hinokitiol (DMSO, 5 µM, 10 µM and 20 µM) for 24 h. ( D ) Representative Western blot showing the expression of UHRF1 in HK-2 cells treated with Hinokitiol (DMSO, 5 µM, 10 µM and 20µM) for 24 h. ( E ) The RNA expression level of UHRF1 was assessed by real-time polymerase chain reaction in HK-2 cells treated with Hinokitiol (DMSO, 5 µM and 10 µM) for 48 h. ( F ) Representative Western blot showing the expression of UHRF1 in HK-2 cells treated with Hinokitiol (DMSO, 5 µM and 10 µM) for 48 h. ( n = 3 per group, data are expressed as mean ± SD. * *P <0.01, ** *P <0.001)

Article Snippet: Mice were randomly divided into three groups ( n = 6): Sham group, UUO group, and UUO + Hinokitiol group, with Hinokitiol (HY-B2230, MedChemExpress; 25 mg/kg) injected intraperitoneally one day before and three days after ligation, and an equal amount of solvent injected in the Sham and UUO groups.

Techniques: Expressing, Full Display Name, CCK-8 Assay, RNA Expression, Real-time Polymerase Chain Reaction, Western Blot

Journal: Scientific Reports

Article Title: UHRF1 promotes epithelial-mesenchymal transition mediating renal fibrosis by activating the TGF-β/SMAD signaling pathway

doi: 10.1038/s41598-025-86496-9

Figure Lengend Snippet: Hinokitiol could ameliorate TGF-β1–induced EMT and fibrosis by inhibiting UHRF1 in HK-2 cells. ( A ) SNAI1 and COL1A1 mRNA levels in different groups were assessed by real-time polymerase chain reaction. HK-2 cells were treated with TGF-β1 (5 ng/ml) alone or cotreated Hinokitiol (10 µM) for 48 h. ( B ) Representative Western blot showing the expression of N-cadherin, Vimentin, Snail1, α-SMA and Collagen I in different groups. HK-2 cells were treated with TGF-β1 (5 ng/ml) alone or cotreated Hinokitiol (10 µM) for 48 h. ( C ) Representative Western blot showing the expression of P-SMAD3, SMAD3, P-SMAD2 and SMAD2 in different groups. HK-2 cells were treated with Hinokitiol (10 µM) for 48 h alone or followed by TGF-β1 (5 ng/ml) for 1 h. ( n = 3 per group, data are expressed as mean ± SD. * *P <0.01, ** *P <0.001)

Article Snippet: Mice were randomly divided into three groups ( n = 6): Sham group, UUO group, and UUO + Hinokitiol group, with Hinokitiol (HY-B2230, MedChemExpress; 25 mg/kg) injected intraperitoneally one day before and three days after ligation, and an equal amount of solvent injected in the Sham and UUO groups.

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Expressing

Journal: Scientific Reports

Article Title: UHRF1 promotes epithelial-mesenchymal transition mediating renal fibrosis by activating the TGF-β/SMAD signaling pathway

doi: 10.1038/s41598-025-86496-9

Figure Lengend Snippet: Hinokitiol could improve renal fibrosis in UUO mice. ( A ) Experimental design. Red dots indicate the timing of UUO surgery. Mice were sacrificed 7 days after UUO surgery. Grey arrows indicate injections of Hinokitiol (25 mg/kg). ( B ) Representative micrographs showing renal expression of Masson’s trichrome staining, Fibronectin, Collagen III and P-SMAD3 in different groups. Red arrows indicate tubular dilatation. Scale bar = 50 μm. ( C ) Quantitative determination of fibrotic areas and the expression of Fibronectin, Collagen III and P-SMAD3 in different groups. ( D ) Representative Western blot showing the expression of E-cadherin, N-cadherin, Vimentin, Snail1, α-SMA and UHRF1 in different groups. ( E ) Representative Western blot showing the expression of P-SMAD3, SMAD3, P-SMAD2 and SMAD2 in different groups. Numbers (1–3) indicate each individual animal in a given group. ( n = 3 per group, data are expressed as mean ± SD. *P <0.05, * *P <0.01, ** *P <0.001)

Article Snippet: Mice were randomly divided into three groups ( n = 6): Sham group, UUO group, and UUO + Hinokitiol group, with Hinokitiol (HY-B2230, MedChemExpress; 25 mg/kg) injected intraperitoneally one day before and three days after ligation, and an equal amount of solvent injected in the Sham and UUO groups.

Techniques: Expressing, Staining, Western Blot

Journal: Scientific Reports

Article Title: UHRF1 promotes epithelial-mesenchymal transition mediating renal fibrosis by activating the TGF-β/SMAD signaling pathway

doi: 10.1038/s41598-025-86496-9

Figure Lengend Snippet: Hinokitiol could ameliorate renal fibrosis in folic acid–induced nephropathy. ( A ) Experimental design. Red dots indicate the timing of intraperitoneal injection with folic acid (250 mg/kg). Mice were sacrificed at 4 weeks after injection with folic acid. Grey arrows indicate injections of Hinokitiol (25 mg/kg). ( B ) Representative micrographs showing renal expression of Masson’s trichrome staining, Sirius red staining, Fibronectin and Collagen III in different groups. Scale bar = 50 μm. ( C ) Quantitative determination for fibrotic areas, the expression of Fibronectin and Collagen III in different groups. ( D ) Representative Western blot showing the expression of E-cadherin, N-cadherin, Vimentin, α-SMA and UHRF1 in different groups. Numbers (1–3) indicate each individual animal in a given group. ( n = 3 per group, data are expressed as mean ± SD. *P <0.05, ** *P <0.001)

Article Snippet: Mice were randomly divided into three groups ( n = 6): Sham group, UUO group, and UUO + Hinokitiol group, with Hinokitiol (HY-B2230, MedChemExpress; 25 mg/kg) injected intraperitoneally one day before and three days after ligation, and an equal amount of solvent injected in the Sham and UUO groups.

Techniques: Injection, Expressing, Staining, Western Blot